Products & Applications > Collagen Coated Plates
Collagen Coated Plates
Collagen Type 0 Coated Plates for Routine Cell Culture Research.
6, 24 and 96 well cultureware plates coated with Collagen Type 0, suitable for cell culture research purposes. The grade of Collagen Type 0 used to coat this cultureware has been tested to verify its applicability for routine cell culture research using human primary and iPSC-derived cell lines. Jellagen® Collagen Type 0 has been shown to promote cellular attachment and proliferation. Cell lines that have been cultured successfully on Collagen Type 0 include, but are not limited to:
Mesenchymal Stem Cells (MSC’s), fibroblasts, hepatocytes, endothelial cells, keratinocytes, chondrogenic
progenitor cells, Urine Derived Stem Cells (UDC’s), cardiomyocytes, ovarian cancer cells, iPSC-derived microglia
*Bespoke formats available on request and subject to volume
This product is for research purposes only.
|Innovative||Offers a viable alternative to mammalian and synthetic reagents with the below features and benefits.|
|Non-mammalian & disease vector free||Marine-sourced Collagen Type 0, avoiding the ethical and safety concerns associated with mammalian collagen.|
|Compatible with all existing cell culture protocols||Like-for-like substitute for existing collagens in cell culture offering a matrix that promotes cell adhesion, proliferation and cell functionality.|
|Batch to batch consistency||Offers improved research productivity allowing security of product consistency and reproducible results.|
|Sequence homology to collagen type I||Universal applications for multiple cell types e.g. human primary and stem cell-derived.|
|Manufactured according to ISO13485||
Follows a quality controlled manufacturing process
producing a consistent coating on the surface of tissue culture treated plates.
|Individually packed||Easy to use and store with a shelf life of 2 years. Uniquely individually packed plates that help reduce waste.|
|PRODUCT INFORMATION||JELLAGEN COATED PLATES|
|Format||6, 24 & 96 well plates - flat bottom|
|Quantity per pack||5|
|Collagen used||Marine-sourced Collagen Type 0|
|Coating concentration||10μg/cm² (+/- 3.0)|
|Storage||Keep at room temperature|
|Culture type||Epithelial cells, fibroblasts, chondrocytes, hepatocytes, keratinocytes, cardiomyocytes, urine derived stem cells, mesenchymal stem cells|
|Serum level||Serum free|
|Shelf life||24 months from date of manufacture|
|Plate polymer||Tissue culture treated, polystyrene and non-pyrogenic|
|Shipping conditions||Room temperature|
6 well, flat bottom plates.
96 well, flat bottom plates.
24 well, flat bottom plates.
Bespoke sizing also offered
Mycoplasma: The grade of Jellagen® jellyfish collagen used to coat this cultureware has been tested to be Mycoplasma negative
Storage/Stability: Room Temperature – Heating above 40 ºC is not recommended. Store in a cool, dry place. The stability of the product is under evaluation.
Precautions and disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. The procedure below is provided as a guideline, but the onus is on the end-user to tailor the conditions of their experiment to their needs and that of their cell line.
Preparation and seeding
Note: Temperature, pH, gas exchange and cell concentration can affect the rate and efficiency of cell seeding. Optimum seeding rate depends on the type of cell being cultured.
1. Using aseptic technique, remove the coated plate from the packaging in a laminar flow environment.
2. Prepare the plates by washing the wells with the selected tissue culture medium.
Note: Take care to avoid contact with the collagen coating during washing with utensils.
3. Suspend cells at desired concentration in media and dispense sufficient volume of cell solution into the well.
4. Transfer to a 37ºC incubator for about 1 – 2 hours to allow for initial cell attachment.
5. After 24 hours, remove the plate from the incubator and check for cell attachment. Additional testing may be required to optimise the time it takes for the cells to attach.
Changing the media
Change the media 12 to 24 hours after the initial seeding. The frequency of changes will be determined by cell type, cell attachment efficiency, pH utilisation of medium nutrients available to cultures.
Harvesting of cells
Note: Digestion with proteases such as trypsin, papain (cysteine protease)1 or collagenase are suitable methods of releasing cells from the Jellagen Coated Plates.
The strength of the attachment of the cells to the collagen scaffolds will vary from cell line to cell line. The enzyme concentration and digestion time will vary depending upon the activity of the enzyme and the confluence of the cells. Collagenase and/or trypsin may be the preferred method. If using papain, the following method is suggested:
1. Prepare enzyme buffer solution (20mM NaAc pH 6.8, 1mM EDTA, 2mM DTT, 330μg/ml papain)
2.Washing the coated plate with EDTA-PBS may assist the protease digestion.
3. Aspirate the EDTA-PBS solution from the well.
4.Add sufficient dissociation solution to the well.
5. Transfer to a 37ºC incubator. Periodically check for cell detachment.
6. Once the cells have fully detached, remove the cells and dispense in a centrifuge tube.
7. Centrifuge the cells as required.
1. Eun Song., So Yeon Kim., Taehoon Chun., Hyun-Jung Byun. & Young Moo Lee. 2006. Collagen scaffolds derived from a marine source and their biocompatibility. Biomaterials 2. 2951–2961
COATED FLASK PROTOCOL
Cell culture flasks are widely used to expand cells in the lab. Our coated flask protocol allows any size or shape of flask to be coated with Jellagen material, offering increased proliferation and growth.
Suitable for 2D Cell Culture
Materials and Reagents
- Jellagen Research Grade Collagen
- Phosphate Buffered Saline (PBS)
- Cell culture flask (eg. T-75)
- Incubator (37oC)
- Sterile Flow Cabinet
- Centrifuge collagen to reduce bubble and foam formation.
- Calculate the volume of coating solution required:
- Prepare the coating solution by adding collagen to PBS at a ratio of 1:75.
- Add the diluted collagen solution to the flask.
- Swirl the solution so that all the growth surface is covered.
- Incubate for 3 hours at 37oC
- Remove form the incubator and pour out any excess liquid.
- Place the flask, with the lid removed, into a sterile flow cabinet.
- Leave overnight to dry.
- Once dry the flasks are ready to use.
PLEASE NOTE: For even coating ensure the collagen solution covers the whole growth surface.
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